A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1Eric D. Routh *1, Steven D. Creacy *2, Peter E. Beerbower 3, Steven A. Akman 4, James P. Vaughn 1, Philip J. Smaldino 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
G4 Resolvase1 binds to G-quadruplex (G4) structures with the tightest reported affinity for a G4-binding protein and represents the majority of the G4-DNA unwinding activity in HeLa cells. We describe a novel protocol that harnesses the affinity and ATP-dependent unwinding activity of G4-Resolvase1 to specifically purify catalytically active recombinant G4R1.
