Accedi

Tyrolean Cancer Research Institute (TKFI)

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

Maximilian Boesch^*1,2,3, Elisabeth Hoflehner^2,3, Dominik Wolf^2,3,4, Guenther Gastl², Sieghart Sopper^*2,3

¹Institute of Immunobiology, Kantonsspital St. Gallen, ²Internal Medicine V, Medical University of Innsbruck, ³Tyrolean Cancer Research Institute (TKFI), ⁴Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology, University Clinic Bonn (UKB)

Methods allowing the characterization and isolation of stem cell populations from biological samples are critical for the advance of stem cell-targeted treatments in cancer and beyond. Here, we provide a detailed protocol for cancer stem cell isolation using the dye-triggered side population phenotype.

Developmental Biology

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Roman Crazzolara¹, Gabriele Kropshofer¹, Michael Steurer², Sieghart Sopper^2,3, Wolfgang Schwinger⁴

¹Department of Pediatrics, Medical University Innsbruck, ²Department of Internal Medicine V (Hematology & Oncology), Medical University Innsbruck, ³Tyrolean Cancer Research Institute, ⁴Department of Pediatrics, Medical University Graz

Quantification of donor-derived cells is required to monitor engraftment after stem cell transplantation in patients with hemoglobinopathies. A combination of flow cytometry-based cell sorting, colony formation assay, and subsequent analysis of short tandem repeats may be used to assess the proliferation and differentiation of progenitors in the erythroid compartment.