Accedi

University Hospital Zurich, University of Zurich

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Florian S. Frueh^1,2, Thomas Später¹, Claudia Scheuer¹, Michael D. Menger¹, Matthias W. Laschke¹

¹Institute for Clinical and Experimental Surgery, Saarland University, ²Division of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich

We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.

Bioengineering

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Lisa A. Krattiger¹, Maria Mitsi², Benjamin R. Simona², Martin Ehrbar¹

¹Department of Obstetrics, University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

An in vitro model of the bone marrow vascular niche is established by seeding mesenchymal and endothelial cells onto pre-cast 3D PEG hydrogels. The endothelial networks, ECM components, and ALP activity of the niches vary depending on the growth factor used. The platform can be used for advanced cancer models.

Medicine

The Combination of Mechanically Isolated Stromal Vascular Fraction and Fibrin Hydrogel: A Processing Protocol

Pablo Pfister^*1, Gregory Reid^*2, Tabea Breckwoldt², Mauro Vasella², Ann-Kathrin Seitz², Seung Yong Song³, Bong-Sung Kim²

¹Department of Surgery, Triemli City Hospital Zurich, ²Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich, ³Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine

Mechanically isolated stromal vascular fraction (SVF) in combination with a fibrin hydrogel offers an easy and efficient carrier for viable adipose-derived stromal cells for various indications, including tissue engineering and or wound healing purposes. Here, we present the preparation of a mechanical SVF (mSVF)-fibrin hydrogel construct for translational research and clinical application.