Accedi

University of the South

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Thea M. Edwards^1,2, Howard E. Morgan^2,3, Coralia Balasca⁴, Naveen K. Chalasani⁵, Lauren Yam^4,6, Alison M. Roark⁴

¹Department of Biology, University of the South, ²School of Biological Sciences, Louisiana Tech University, ³School of Medicine, Louisiana State University Health Sciences Center, ⁴Department of Biology, Furman University, ⁵Department of Computer Science, Louisiana Tech University, ⁶Clemson University

This article presents an optimized yeast estrogen screen for quantifying ligands in Personal Care Products (PCPs) that bind estrogen receptors alpha (ERα) and/or beta (ERβ). The method incorporates two colorimetric substrate options, a six-day refrigerated incubation for use in undergraduate courses, and statistical tools for data analysis.