Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free ConditionsJaroslav Slamecka 1, Javier Laurini 2, Troy Shirley 1, Simon Philipp Hoerstrup 3, Benedikt Weber 3,4,5, Laurie Owen 1, Steven McClellan 1
1Mitchell Cancer Institute, University of South Alabama, 2College of Medicine, University of South Alabama, 3Institute for Regenerative Medicine, University of Zurich, 4Department of Dermatology, University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
