The University of Dublin

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Measurement & Analysis of the Temporal Discrimination Threshold Applied to Cervical Dystonia

Rebecca B Beck^*1, Eavan M McGovern^*1,2,3, John S Butler⁴, Dorina Birsanu¹, Brendan Quinlivan¹, Ines Beiser^1,2,3, Shruti Narasimham¹, Sean O'Riordan^2,3, Michael Hutchinson^2,3, Richard B Reilly^1,5

¹School of Engineering, Trinity College Dublin, The University of Dublin, ²Department of Neurology, St. Vincent's University Hospital, ³School of Medicine and Medical Sciences, University College Dublin, ⁴School of Mathematical Sciences, Dublin Institute of Technology, ⁵School of Medicine Trinity College Dublin, The University of Dublin

Methods for the measurement and analysis of the temporal discrimination threshold are presented, and its application to the study of the pathogenesis of cervical dystonia are discussed.

Immunology and Infection

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Seónadh O’Leary¹, Ahmad Z. Bahlool^1,2, Gemma O’Connor², Sally-Ann Cryan^2,3,4, Joseph M. Keane¹, Mary P. O’Sullivan¹

¹Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, ²School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), ³SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, ⁴SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland

Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.

Immunology and Infection

The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood

Anjali S. Yennemadi¹, Joseph Keane¹, Gina Leisching¹

¹TB Immunology Group, Department of Clinical Medicine, Trinity Translational Medicine Institute, St James's Hospital, Trinity College Dublin, The University of Dublin

Here, we provide a reliable approach for isolating low- and normal-density neutrophils from whole blood using magnetic isolation (negative selection) and discontinuous density gradient medium. It ensures untouched isolation of high-purity cells (≥93%), facilitating accurate downstream analysis of neutrophil subpopulations, crucial for understanding their roles in health and disease.