Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein InteractionsMyriam Jaraíz-Rodríguez 1, Ana González-Sánchez 1,2, Laura García-Vicente 1, Jose M. Medina 1, Arantxa Tabernero 1
1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, 2Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast
This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.
