Accedi

Kaohsiung Medical University Hospital

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Yi-Chen Lee^1,2, Shui-Chin Lu³, Yu-Lin Hsieh^1,2

¹Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Medical Research, Kaohsiung Medical University Hospital, ³Department of Medical Research, Ultrastructural Laboratory, Kaohsiung Medical University Hospital

This study establishes an experimental model of pure small fiber neuropathy with resiniferatoxin (RTX). A unique dose of RTX (50 µg/kg) is optimal for developing a small fiber neuropathy model that mimics patient characteristics and could help investigate the nociceptive molecular significance underlying neuropathic pain.

Medicine

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats

Hsin-Ting Lin^1,2, Yi-Lin Shiou¹, Wun-Jyun Jhuang¹, Hsiang-Chun Lee^1,3,4,5,6

¹Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, ²Hematology and Oncology, China Medical University Hospital, ³Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, ⁴Center for Lipid Biosciences, Kaohsiung Medical University Hospital, ⁵Lipid Science and Aging Research Center, Kaohsiung Medical University, ⁶Institute/Center of Medical Science and Technology, National Sun Yat-sen University

Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.