Accedi

Oxford University

4 ARTICLES PUBLISHED IN JoVE

Biochemistry

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Mikhail A. Galkin¹, Aidan N. Russell², Steven B. Vik³, Richard M. Berry², Robert R. Ishmukhametov²

¹First Pavlov State Medical University, ²Clarendon Laboratory, Department of Physics, Oxford University, ³Department of Biological Sciences, Southern Methodist University

Here we present two ultrafast protocols for reconstitution of membrane proteins into fusogenic proteoliposomes, and fusion of such proteoliposomes with target lipid bilayers for detergent-free delivery of these membrane proteins into the postfusion bilayer. The combination of these approaches enables fast and easily controlled assembly of complex multi-component membrane systems.

Chemistry

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Alice Douangamath^*1,2, Ailsa Powell^*1,2, Daren Fearon^*1,2, Patrick M. Collins^1,2, Romain Talon^1,2,3, Tobias Krojer^3,4, Rachael Skyner^1,2, Jose Brandao-Neto^1,2, Louise Dunnett^1,2, Alexandre Dias^1,2, Anthony Aimon^1,2,3, Nicholas M. Pearce^1,3, Conor Wild^3,5, Tyler Gorrie-Stone¹, Frank von Delft^1,2,3,4,6

¹Diamond Light Source Ltd, Harwell Science and Innovation Campus, ²Research Complex at Harwell, Harwell Science and Innovation Campus, ³Structural Genomics Consortium, University of Oxford, ⁴Centre for Medicines Discovery, University of Oxford, ⁵Oxford Protein Informatics Group, Department of Statistics, Oxford University, ⁶Department of Biochemistry, University of Johannesburg

This paper describes the complete XChem process for crystal-based fragment screening, starting from applying for access and all subsequent steps to data dissemination.

iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology

Hans Wienk¹, Lucia Banci², Susan Daenke³, Eva Pereiro⁴, Harald Schwalbe⁵, David Ian Stuart⁶, Manfred S. Weiss⁷, Anastassis Perrakis¹

¹Division of Biochemistry and Oncode Institute, The Netherlands Cancer Institute, Amsterdam, ²CERM/CIRMMP, University of Florence, ³Instruct-ERIC, Oxford University, ⁴MISTRAL beamline, Alba Light Source, ⁵Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, ⁶Division of Structural Biology, Oxford University, ⁷Macromolecular Crystallographyy, Helmholtz-Zentrum Berlin

iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology

Advanced Methods In Parkinson’s Disease Research

Nora Bengoa-Vergniory^1,2

¹Oxford University, ²Achucarro Basque Centre for Neurosience, Ikerbasque, UPV/EHU

Advanced Methods In Parkinson’s Disease Research