Accedi

Wenzhou Medical College

2 ARTICLES PUBLISHED IN JoVE

Biology

A Method for Islet Transplantation to the Omentum in Mouse

Ying Lu^*1, Shangyou Zou^*1, Suzanne Bertera^*2, Rita Bottino², David K.C. Cooper³, Zhengzhao Liu¹, Yi Huang¹, Chengjun Wang¹, Chungu Hong¹, Tian He¹, Hancheng Zhang¹, Qin Huo⁴, Hongxing Fu⁵, Zhiming Cai¹, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen University Health Science Center, ²Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, ³Xenotransplantation Program/Department of Surgery, The University of Alabama at Birmingham, ⁴College of Life Science and Oceanography, Shenzhen University, ⁵School of Pharmcy, Wenzhou Medical College

A method for the omental transplantation of islets in a mouse is introduced. The isolated islets are mixed with hydrogel and the mixture is placed into the omental pouch of a diabetic mouse. Then, the blood glucose is monitored, and immuno-histochemical analysis is performed.

Medicine

Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

Yuanzheng Peng^*1, Zhicheng Zou^*1, Jiao Chen¹, Hancheng Zhang¹, Ying Lu¹, Rito Bittino², Hongxing Fu³, David K. C. Cooper⁴, Shan Lin¹, Mengtao Cao¹, Yifan Dai⁵, Zhiming Cai¹, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Health Science Center, ²Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, ³School of Pharmaceutical Sciences, Wenzhou Medical University, ⁴Xenotransplantation Program/Department of Surgery, University of Alabama at Birmingham, ⁵Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University

In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.