Accedi

Dongfang Hospital of Beijing University of Chinese Medicine

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Xuan Guo^1,2, Yiqing Zhao^1,3, Hieu Nguyen¹, Tonghua Liu², Zhenghe Wang^1,3, Hua Lou^1,3,4

¹Department of Genetics and Genome Sciences, Case Western Reserve University, ²Department of Endocrinology, Dongfang Hospital of Beijing University of Chinese Medicine, ³Case Comprehensive Cancer Center, Case Western Reserve University, ⁴Center for RNA Science and Therapeutics, Case Western Reserve University

An in situ hybridization (ISH) protocol that uses short antisense oligonucleotides to detect alternative pre-mRNA splicing patterns in mouse brain sections is described.