Accedi

São Paulo State University (UNESP)

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

Marlise I. Klein¹, Jin Xiao^1,2, Arne Heydorn³, Hyun Koo^1,4

¹Center for Oral Biology, University of Rochester Medical Center, ²State Key Laboratory of Oral Diseases, Sichuan University, ³Department of General Medicine, Glostrup Hospital, Glostrup, Denmark, ⁴Department of Microbiology and Immunology, University of Rochester Medical Center

Biofilms formed on tooth surfaces are highly complex and exposed to constant innate and exogenous environmental challenges, which modulate their architecture, physiology and transcriptome. We developed a toolbox to examine the composition, structural organization and gene expression of oral biofilms, which can be adapted to other areas of biofilm research.

Developmental Biology

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Amanda B.T. Hill^1,2, Jonathan E.B.T. Hill¹, Fabiana F. Bressan³, Maria Angélica Miglino⁴, Joaquim M. Garcia¹

¹The School of Agricultural and Veterinary Studies (FCAV), Jaboticabal, São Paulo State University (UNESP), ²CRRF, Université de Montréal, ³FZEA, University of São Paulo, ⁴FMVZ, University of São Paulo

Herein, we describe a method for the isolation, expansion, and differentiation of mesenchymal stem cells from canine ovarian tissue.

Chemistry

Niobium Oxide Films Deposited by Reactive Sputtering: Effect of Oxygen Flow Rate

Silvia L. Fernandes¹, Lucas J. Affonço², Roberto A. R. Junior², José H. D. da Silva², Elson Longo¹, Carlos F. de O. Graeff²

¹Chemistry Department, Federal University of São Carlos (UFSCAR), ²Physics Department, School of Sciences, São Paulo State University (UNESP)

Here, we present a protocol for niobium oxide films deposition by reactive sputtering with different oxygen flow rates for use as an electron transport layer in perovskite solar cells.

JoVE Core

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Sabrina M. Ribeiro^*1, Érick D. O. Fratucelli^*1, Júlia M. Fernandes^*2, Paula C. P. Bueno^2,3, Alberto José Cavalheiro², Marlise I. Klein¹

¹Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), ²Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), ³Faculty of Pharmaceutical Sciences of Ribeiraão Preto (FCFRP-USP), Department of Physics and Chemistry, University of São Paulo

Natural products represent promising starting points for the development of new drugs and therapeutic agents. However, due to the high chemical diversity, finding new therapeutic compounds from plants is a challenging and time-consuming task. We describe a simplified approach to identify antimicrobial and antibiofilm molecules from plant extracts and fractions.

Immunology and Infection

Curcuminoid-Mediated Antimicrobial Photodynamic Therapy on a Murine Model of Oral Candidiasis

Ewerton Garcia de Oliveira Mima¹, Ana Cláudia Pavarina¹, Cláudia Carolina Jordão¹, Sâmmea Martins Vieira², Lívia Nordi Dovigo²

¹Department of Dental Materials and Prosthodontics, School of Dentistry, Araraquara, São Paulo State University (UNESP), ²Department of Social Dentistry, School of Dentistry, Araraquara, São Paulo State University (UNESP)

This protocol describes the application of antimicrobial Photodynamic Therapy (aPDT) in a murine model of oral candidiasis. aPDT was performed using a water-soluble mixture of curcuminoids and blue LED light.