Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdUZuzana Kocsisova 1,2, Ariz Mohammad 2, Kerry Kornfeld 1, Tim Schedl 2
1Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics, Washington University School of Medicine, St. Louis, Missouri
An imaging-based method is described that can be used to identify S-phase and analyze cell cycle dynamics in the C. elegans hermaphrodite germline using the thymidine analog EdU. This method requires no transgenes and is compatible with immunofluorescent staining.
