Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of LymphomaGuo Hong *1,2, Shuangyi Fan *3, The Phyu 3, Priyanka Maheshwari 2, Michal Marek Hoppe 2, Hoang Mai Phuong 2, Sanjay de Mel 4, Michelle Poon 4, Siok-Bian Ng 2,3, Anand D. Jeyasekharan 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.
