Accedi

Univ Montpellier

3 ARTICLES PUBLISHED IN JoVE

Medicine

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Solja Kalha¹, Alison Kuony¹, Frederic Michon^1,2

¹Helsinki Institute of Life Science, Institute of Biotechnology, University of Helsinki, ²School of Medicine and Institute for Science and Technology in Medicine, Keele University

This protocol describes a method to inflict an abrasion to the ocular surface of the mouse, and to follow the wound healing process thereafter. The protocol takes advantage of an ocular burr to partially remove the surface epithelium of the eye in anaesthetized mice.

Immunology and Infection

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Michel Jourdan¹, Hugues de Boussac¹, Elena Viziteu¹, Alboukadel Kassambara¹, Jérôme Moreaux^1,2,3

¹IGH, CNRS, Univ Montpellier, ²Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology, CHU Montpellier, ³UFR de Médecine, Univ Montpellier

Using multi-step culture systems, we report an in vitro B cell to plasma cell differentiation model.

Biology

Zebrafish Corneal Wound Healing: From Abrasion to Wound Closure Imaging Analysis

Kaisa Ikkala¹, Sini Raatikainen¹, Frederic Michon^1,2

¹Institute of Biotechnology, HiLIFE, University of Helsinki, ²Institute for Neurosciences of Montpellier, Univ Montpellier

This protocol focuses on damaging the ocular surface of zebrafish through abrasion to assess the subsequent wound closure at the cellular level. This approach exploits an ocular burr to partly remove the corneal epithelium and uses scanning electron microscopy to track changes in cell morphology during wound closure.