Accedi

ACEA Biosciences, Inc

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Biao Xi¹, Robert Berahovich², Hua Zhou², Shirley Xu², Yuehua Wei², Jasper Guan², Hizkia Harto², Jian Guan², Lijun Wu², David Santa Ana^1,3, Fabio Cerignoil¹, Brandon Lamarche¹, Yama A. Abassi¹, Vita Golubovskaya²

¹ACEA Biosciences, Inc, ²ProMab Biotechnologies. Inc, ³ACEA Therapeutics

We describe a quantitative real-time in vitro cytolysis assay system to evaluate the potency of chimeric antigen receptor T cells targeting liquid and solid tumor cells. This protocol can be extended to assess other immune effector cells, as well as combination treatments.