Accedi

Parc Científic de Barcelona

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development

Vanessa Gil^1,2,3,4, José Antonio Del Río^1,2,3,4

¹Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, ²Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, ³Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), ⁴Institute of Neuroscience, University of Barcelona

Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.

Developmental Biology

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Federica Mangione^1,2, Enrique Martin-Blanco¹

¹Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Científic de Barcelona, ²The Francis Crick Institute

This protocol is designed for the imaging and analysis of the dynamics of cell orientation and tissue growth in the Drosophila abdominal epithelia as the fruit fly undergoes metamorphosis. The methodology described here can be applied to the study of different developmental stages, tissues, and subcellular structures in Drosophila or other model organisms.