Accedi

Shandong Provincial Hospital affiliated to Shandong University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

¹Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham

We present three novel and more efficient protocols for differentiating human induced pluripotent stem cells into cardiomyocytes, endothelial cells, and smooth muscle cells and a delivery method that improves the engraftment of transplanted cells by combining cell injection with patch-mediated cytokine delivery.

Immunology and Infection

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

Howard W. Davidson¹, Joseph Ray Cepeda², Nitin S. Sekhar², Junying Han², Ling Gao³, Tomasz Sosinowski¹, Li Zhang²

¹Barbara Davis Center for Childhood Diabetes, University of Colorado Denver, ²Department of Medicine, Endocrinology, Diabetes & Metabolism, Baylor College of Medicine, ³Scientific Center, Shandong Provincial Hospital affiliated to Shandong University

This article describes a protocol for the generation of antigen-specific CD8 T cells, and their expansion in vitro, with the aim of yielding high numbers of functional T cells for use in vitro and in vivo.