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Estación Experimental del Zaidín (CSIC)

1 ARTICLES PUBLISHED IN JoVE

Genetics

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Rafael J. L. Morcillo¹, Achen Zhao^1,2, María I. Tamayo-Navarrete³, José M. García-Garrido³, Alberto P. Macho¹

¹Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, ²University of Chinese Academy of Sciences, ³Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC)

Here, we present a versatile method for tomato root transformation followed by inoculation with Ralstonia solanacearum to perform straightforward genetic analysis for the study of bacterial wilt disease.