Accedi

University of California San Francisco and University of California Berkeley

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Jonathon M. Muncie^1,2, Roberto Falcón-Banchs¹, Johnathon N. Lakins², Lydia L. Sohn^1,3, Valerie M. Weaver^2,4,5,6

¹Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, ²Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California San Francisco, ³Department of Mechanical Engineering, University of California Berkeley, ⁴Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, ⁵UCSF Comprehensive Cancer Center, Helen Diller Family Cancer Research Center, University of California San Francisco, ⁶Department of Anatomy, Department of Bioengineering and Therapeutic Sciences, and Department of Radiation Oncology, University of California San Francisco

Extracellular matrix ligands can be patterned onto polyacrylamide hydrogels to enable the culture of human embryonic stem cells in confined colonies on compliant substrates. This method can be combined with traction force microscopy and biochemical assays to examine the interplay between tissue geometry, cell-generated forces, and fate specification.

Bioengineering

Simple, Affordable, and Modular Patterning of Cells using DNA

Katelyn A. Cabral¹, David M. Patterson², Olivia J. Scheideler¹, Russell Cole³, Adam R. Abate^4,5,6, David V. Schaffer^7,8, Lydia L. Sohn⁹, Zev J. Gartner^2,6,10

Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion_.

Bioengineering

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Andre Lai^*1, Rachel Rex^*2, Kristen L. Cotner¹, Alan Dong³, Michael Lustig^1,3, Lydia L. Sohn^1,2

¹Graduate Program in Bioengineering, University of California, Berkeley and University of California, San Francisco, ²Department of Mechanical Engineering, University of California, Berkeley, ³Department of Electrical Engineering and Computer Sciences, University of California, Berkeley

Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.