Simple Lithography-Free Single Cell Micropatterning using Laser-Cut StencilsSoah Lee *1,2,3, Huaxiao Yang *1,2,3, Caressa Chen *1,2,3, Sneha Venkatraman 1,2,3, Adrija Darsha 1,2,3, Sean M. Wu 1,2,3, Joseph C. Wu 1,2,3, Timon Seeger 1,2,3,4,5
1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 4Department of Medicine III, University Hospital Heidelberg, 5German Centre for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim
This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.
