Accedi

VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS)

2 ARTICLES PUBLISHED IN JoVE

Education

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Robert Szulcek¹, Harm Jan Bogaard¹, Geerten P. van Nieuw Amerongen²

¹Department of Pulmonary Diseases, Institute for Cardiovascular Research, VU University Medical Center, ²Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center

This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.

Immunology and Infection

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

Xue D. Manz¹, Hugo J. Albers^2,3, Petr Symersky⁴, Jurjan Aman¹, Andries D. van der Meer³, Harm Jan Bogaard¹, Robert Szulcek^1,5,6

¹Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), ²BIOS Lab-on-a-Chip group, University of Twente, ³Applied Stem Cell Technologies Group, University of Twente, ⁴Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, ⁵Institute of Physiology, Charité-Universitätsmedizin, ⁶German Heart Center

We present an in vitro vascular disease model to investigate whole blood interactions with patient-derived endothelium. This system allows the study of thrombogenic properties of primary endothelial cells under various circumstances. The method is especially suited to evaluate in situ thrombogenicity and anticoagulation therapy during different phases of coagulation.