Kidney Organoid Generation at the Air-Liquid InterfaceAshwani Kumar Gupta 1,2, David Z. Ivancic 1, Bilal A. Naved 1,3, Jason A. Wertheim 1,2,3,4,5,6, Leif Oxburgh 7
1Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, 2Department of Surgery, Northwestern University Feinberg School of Medicine, 3Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, 4Simpson Querrey Institute for BioNanotechnology, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Department of Surgery, Jesse Brown VA Medical Center, 7The Rogosin Institute
This protocol describes asynchronous mixing of human embryonic stem cells derived kidney progenitors at the air-liquid interface to efficiently generate kidney organoids.
