Live Animal Imaging and Cell Sorting Methods for Investigating Neurodegeneration in a C. elegans Excitotoxic Necrosis ModelZelda Z Mendelowitz *1,2, Adem Idrizi *1, Itzhak Mano 1,2
1Cellular, Molecular, and Biomedical Science, CUNY School of Medicine, The City University of New York (CUNY), 2PhD Program in Biology, The CUNY Graduate Center, The City University of New York (CUNY)
In a C. elegans excitotoxicity model, this protocol employs in vivo imaging to analyze the regulation of necrotic neurodegeneration, the effect of genes encoding candidate mediators, and involvement of mitochondria. Cell dissociation and sorting is used to specifically obtain at-risk neurons for cell-specific transcriptomic analysis of neurodegeneration and neuroprotection mechanisms.
