The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery StudiesBram Meijlink 1, Ilya Skachkov 1, Antonius F. W. van der Steen 1,2, Nico de Jong 1,2, Klazina Kooiman 1
1Department of Biomedical Engineering, Thoraxcenter, Erasmus MC University Medical Center Rotterdam, 2Laboratory of Acoustical Wavefield Imaging, Faculty of Applied Sciences, Delft University of Technology
This protocol describes three methods on how to obtain and use 5 to 8-day old chicken embryos and their chorioallantoic membrane (CAM) as an in vivo model to study contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery.
