High-Throughput Transcriptome Analysis for Investigating Host-Pathogen InteractionsAndré Nicolau Aquime Gonçalves 1,2, Vanessa Escolano Maso 3, Ícaro Maia Santos de Castro 2,3, Amanda Pereira Vasconcelos 3, Rodrigo Luiz Tomio Ogava 2,3, Helder I Nakaya 2,3,4
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School, University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, 4Hospital Israelita Albert Einstein
The protocol presented here describes a complete pipeline to analyze RNA-sequencing transcriptome data from raw reads to functional analysis, including quality control and preprocessing steps to advanced statistical analytical approaches.
