Accedi

Rutgers-The State University of New Jersey

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

Peilin Zheng^*1,2, Grant Bertolet^*1,2,3, Yuhui Chen^1,2, Shengjian Huang^1,2, Dongfang Liu^1,2,3

¹Center for Human Immunobiology, Texas Children's Hospital, ²Department of Pediatrics, Baylor College of Medicine, ³Department of Pathology and Immunology, Baylor College of Medicine

We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

Cancer Research

Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Minh Ma^1,2, Saiaditya Badeti¹, James K. Kim¹, Dongfang Liu^1,3

¹Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, ²Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, ³Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

Here, we present a method to expand peripheral blood natural killer (PBNK), NK cells from liver tissues, and chimeric antigen receptor (CAR)-NK cells derived from peripheral blood mononuclear cells (PBMCs) or cord blood (CB). This protocol demonstrates the expansion of NK and CAR-NK cells using 221-mIL-21 feeder cells in addition to the optimized purity of expanded NK cells.