Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp SpectroscopyL. Gardini 1,2, A. V. Kashchuk 2,3, F. S. Pavone 1,2,3, M. Capitanio 2,3
1National Institute of Optics, National Research Council, 2LENS, European Laboratory for Non-Linear Spectroscopy, 3Physics Department, University of Florence
Presented here is a comprehensive protocol to perform ultrafast force-clamp experiments on processive myosin-5 motors, which could be easily extended to the study of other classes of processive motors. The protocol details all the necessary steps, from the setup of the experimental apparatus to sample preparation, data acquisition and analysis.
