Accedi

Kyoto Institute of Technology

2 ARTICLES PUBLISHED IN JoVE

Biology

Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

Robert M. Cooper^*1, Taishi Tonooka^*1,2, Andriy Didovyk^*1,3, Jeff Hasty^1,4,5

¹Biocircuits Institute, University of California, San Diego, ²Present address, Kyoto Institute of Technology, ³Present address, Vertex Pharmaceuticals, ⁴Department of Bioengineering, University of California, San Diego, ⁵Molecular Biology Section, University of California, San Diego

This protocol describes a rapid and simple method to produce bacterial lysate for cell-free gene expression, using an engineered strain of Escherichia coli and requiring only standard laboratory equipment.

Genetics

Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains

Kaori Nishimura^*1, Miho Asaoka^*2, Yurina Sakamaki^*2, Tatsuya Fukumoto^*3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center, Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, ³Research Center of Genetic Resources, National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

A long-term preservation method for Drosophila strains as an alternative to the frequent transfer of adult flies to fresh food vials is highly desirable. This protocol describes the cryopreservation of Drosophila primordial germ cells and strain revival via their transplantation to agametic host embryos.