Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell CulturesCindy Bokobza *1, Alice Jacquens *1,2, Manuela Zinni 1, Valérie Faivre 1, Jennifer Hua 1, David Guenoun 1, Caroline Userovici 1, Shyamala Mani 1, Vincent Degos 1,2, Pierre Gressens 1, Juliette Van Steenwinckel 1
1NeuroDiderot, Inserm UMR-1141, Hôpital Robert Debré 48, Université de Paris, 2Department of anesthesia and critical care, APHP-Sorbonne university
Primary microglia cultures are commonly used to evaluate new anti-inflammatory molecules. The present protocol describes a reproducible and relevant method to magnetically isolate microglia from neonate pups.
