Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T CellsKasper Mølgaard *1, Anne Rahbech *1, Özcan Met 1, Inge Marie Svane 1, Per thor Straten 1,3, Claus Desler 2, Marlies J. W. Peeters 1
1National Center for Cancer Immune Therapy, Department of Oncology, University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging, University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group, University of Copenhagen
Metabolic adaptation is fundamental for T cells as it dictates differentiation, persistence, and cytotoxicity. Here, an optimized protocol for monitoring mitochondrial respiration in ex vivo cytokine-differentiated human primary T cells is presented.
