Accedi

Furman University

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

Kellie N. Bingham^*1, Megan D. Lee^*1, Jason S. Rawlings¹

¹Department of Biology, Furman University

Flow cytometry can be utilized to assess the state of chromatin within T cells. This protocol allows scientists to interpret evidence of chromatin decondensation during T cell activation demonstrated by an increase in the mean florescence intensity (MFI) of fluorescent Histone H3 antibodies

JoVE Journal

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Thea M. Edwards^1,2, Howard E. Morgan^2,3, Coralia Balasca⁴, Naveen K. Chalasani⁵, Lauren Yam^4,6, Alison M. Roark⁴

¹Department of Biology, University of the South, ²School of Biological Sciences, Louisiana Tech University, ³School of Medicine, Louisiana State University Health Sciences Center, ⁴Department of Biology, Furman University, ⁵Department of Computer Science, Louisiana Tech University, ⁶Clemson University

This article presents an optimized yeast estrogen screen for quantifying ligands in Personal Care Products (PCPs) that bind estrogen receptors alpha (ERα) and/or beta (ERβ). The method incorporates two colorimetric substrate options, a six-day refrigerated incubation for use in undergraduate courses, and statistical tools for data analysis.

Education

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Tuguldur T. Odbadrakh¹, Ariel G. Gale¹, Benjamin T. Ball¹, Berhane Temelso², George C. Shields¹

¹Department of Chemistry, Furman University, ²College of Charleston

The atmospheric concentrations of weakly bound molecular clusters can be computed from the thermochemical properties of low energy structures found through a multi-step configurational sampling methodology utilizing a genetic algorithm and semi-empirical and ab initio quantum chemistry.