Accedi

Bristol-Myers Squibb

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Yoshinobu Koguchi¹, Iliana L. Gonzalez¹, Tanisha L. Meeuwsen¹, William L. Miller¹, Daniel P. Haley^1,2, Alice N. Tanibata-Branham³, Keith S. Bahjat^1,4

¹Human Immune Monitoring Laboratory, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, ²Sony Biotechnology, ³Beckman Coulter, Inc. Life Sciences, ⁴Bristol-Myers Squibb

Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.

Genetics

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Adam B. Cornwell¹, Jesse R. Llop¹, Peter Salzman^2,3, Juilee Thakar⁴, Andrew V. Samuelson¹

¹Department of Biomedical Genetics, University of Rochester Medical Center, ²Department of Biostatistics and Computational Biology, University of Rochester Medical Center, ³Non-Clinical Statistics, Bristol-Myers Squibb, ⁴Department of Microbiology and Immunology, University of Rochester Medical Center

Here we describe the Replica Set method, an approach to quantitatively measure C. elegans lifespan/survival and healthspan in a high-throughput and robust manner, thus allowing screening of many conditions without sacrificing data quality. This protocol details the strategy and provides a software tool for analysis of Replica Set data.