biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

immune systems and components

This collection features experimental procedures focused on the isolation, culture, differentiation, and functional analysis of various immune cells. These techniques are designed to investigate different aspects of the immune system and its cellular components. They involve the manipulation and study of immune cells derived from both human and mouse models.

Immune Systems and Components

immune response

This collection presents a range of studies focusing on various aspects of the immune response. This compilation investigates the dynamic interactions between pathogens and immune cells, inflammation induction, immune cell receptor signaling, immune cell activation in response to antibodies, and their impact on other cells. Utilizing advanced techniques like flow cytometry, fluorescence microscopy, and immunoassays, these studies offer valuable insights into the complex immune system and its responses to infections, treatments, and stimuli.

Immune Response

immunopathology

This collection focuses on the intricate interactions between pathogens and the immune systems, shedding light on the aspects of immune responses and disease development. Various infection models are covered to understand host-pathogen interactions better. In vivo and ex vivo models provide insights into the dynamics of inflammation and infection. The collection showcases an array of techniques to decipher the complexities of immunopathology, paving the way for therapeutic interventions and enhancing our understanding of immune-related diseases.

Immunopathology

immunodiagnostics

This collection presents assays and techniques, enabling the examination of immune responses and immunomodulatory effects. It covers various aspects such as bacterial infection detection, cytotoxicity, immune cell interactions, immune synapse formation, cytokine bioactivity, and tissue-specific assessments. By employing advanced imaging techniques, flow cytometry, and fluorescent assays, it fosters an understanding of immune function and the development of immunomodulatory therapies.

Immunodiagnostics

antibody-based technologies

This collection features techniques for antibody production and purification, assays to assess their functionality and specificity, and approaches for their application in imaging and detection, or analysis of various biological samples or conditions. This resource supports researchers in leveraging antibodies for scientific discovery and therapeutic advancements in biomedical research.

Antibody-Based Technologies

immunotherapy

This collection showcases experimental protocols in immunotherapy, highlighting innovative approaches like CAR T-cell production, siRNA treatments, and novel vaccines. Using techniques like CRISPR-Cas9 genetic editing and viral transduction, these protocols explore immune system dynamics to develop treatments for a range of complex diseases. These protocols are key to advancing both the theoretical and practical aspects of immunotherapy.

Immunotherapy

cell isolation and culture

Cell Isolation and Culture

cell differentiation techniques

Cell Differentiation Techniques

assays and functional profiling

Assays and Functional Profiling

isolation of monocytes from whole blood by immunomagnetic negative selection

isolation-monocytes-from-whole-blood-immunomagnetic-negative

Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection

Source: Plaisance-Bonstaff, K., et al. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (2019)
In this video, we demonstrate ...

isolation and culture of mouse kupffer cells

isolation-and-culture-of-mouse-kupffer-cells

Isolation and Culture of Mouse Kupffer Cells

Source: Bourgognon, M., et al. Kupffer Cell Isolation for Nanoparticle Toxicity Testing. J. Vis. Exp. (2015)
In this video, we demonstrate the isolation ...

isolation of macrophages from mouse dorsal root ganglion using mechanical dissociation

isolation-macrophages-from-mouse-dorsal-root-ganglion-using

Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation

Source: Yu, X., et al. Rapid Isolation of Dorsal Root Ganglion Macrophages. J. Vis. Exp. (2019)
This video demonstrates an enzyme-free protocol to isolate ...

isolation of lymphocytes from a mouse peyer&#39;s patch

isolation-of-lymphocytes-from-a-mouse-peyer-s-patch

Isolation of Lymphocytes from a Mouse Peyer&#39;s Patch

Source: Yazicioglu, Y. F., et al. Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer&#39;s ...

isolation of peripheral blood mononuclear cells from human buffy coats

isolation-of-peripheral-blood-mononuclear-cells-from-human-buffy-coats

Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats

Source: Mily, A., et al. Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis ...

isolation and purification of b cells from human peripheral blood

isolation-and-purification-of-b-cells-from-human-peripheral-blood

Isolation and Purification of B Cells from Human Peripheral Blood

Source: Tzeng, S. J. The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of ...

isolation and culture of bone marrow stromal cells from a mouse tibia

isolation-and-culture-of-bone-marrow-stromal-cells-from-a-mouse-tibia

Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia

Source: Maridas, D. E., et al. Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice. J. Vis. ...

flotation-based t cell isolation using buoyancy-activated cell sorting

flotation-based-t-cell-isolation-using-buoyancy-activated-cell-sorting

Flotation-Based T Cell Isolation Using Buoyancy-Activated Cell Sorting

Source: Snow, T., et al. Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using ...

culturing of activated t cells from human peripheral blood mononuclear cells

culturing-activated-t-cells-from-human-peripheral-blood-mononuclear

Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells

Source: M&#248;lgaard, K., et al. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. 
...

isolation of marginating hepatic leukocytes from a mouse liver

isolation-of-marginating-hepatic-leukocytes-from-a-mouse-liver

Isolation of Marginating Hepatic Leukocytes from a Mouse Liver

Source: Sorski, L., et al. Selective Harvesting of Marginating-hepatic Leukocytes. J. Vis. Exp. (2016)
In this video, we demonstrate the isolation of ...

natural killer cell isolation from liver tissue for selective expansion

natural-killer-cell-isolation-from-liver-tissue-for-selective

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion

Source: Ma, M., et al. Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line.&#160;J. Vis. Exp.&#160;(180), ...

ex vivo expansion of natural killer cells from peripheral blood mononuclear cells

ex-vivo-expansion-natural-killer-cells-from-peripheral-blood

Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells

Source: Ma, M., et al. Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line. J. Vis. Exp. (2022).
This ...

isolation of immune regulatory sertoli cells from mouse seminiferous tubules

isolation-immune-regulatory-sertoli-cells-from-mouse-seminiferous

Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules

Source: Bhushan, S. et al., Isolation of Sertoli Cells and Peritubular Cells from Rat Testes.&#160;J. Vis. Exp. (2016).
This video demonstrates the ...

selective isolation of cd4+ v&delta;1+ &gamma;&delta; t cells from human peripheral blood mononuclear cells

selective-isolation-cd4-v1-t-cells-from-human-peripheral-blood

Selective Isolation of CD4+ V&delta;1+ &gamma;&delta; T Cells from Human Peripheral Blood Mononuclear Cells

Source: Welker, C., et al. Isolation and Ex Vivo Culture of V&#948;1+CD4+&#947;&#948; T Cells, an Extrathymic &#945;&#946;T-cell Progenitor. J. Vis. Exp. ...

isolation of cytotoxic neutrophils from tumor-bearing mouse blood

isolation-of-cytotoxic-neutrophils-from-tumor-bearing-mouse-blood

Isolation of Cytotoxic Neutrophils from Tumor-Bearing Mouse Blood

Source: Sionov, R. V., et al., Isolation and Characterization of Neutrophils with Anti-Tumor Properties. J. Vis. Exp. (2015)
In this video, we demonstrate ...

isolation of gingival immune cell network from murine teeth blocks

isolation-of-gingival-immune-cell-network-from-murine-teeth-blocks

Isolation of Gingival Immune Cell Network from Murine Teeth Blocks

Source: Dutzan, N. et al., Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network. J. Vis. Exp. (2016)
This video ...

a technique to isolate primary mast cells from a murine peritoneal cavity

a-technique-to-isolate-primary-mast-cells-from-murine-peritoneal

A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity

Source: Tsvilovskyy, V., et al. Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation ...

magnetic isolation of microglia from mouse pup brains

magnetic-isolation-of-microglia-from-mouse-pup-brains

Magnetic Isolation of Microglia from Mouse Pup Brains

Source: Bokobza, C. et al., Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures. J. Vis. Exp. (2022)
In this video, we ...

harvesting an inguinal draining lymph node from a murine model

harvesting-an-inguinal-draining-lymph-node-from-a-murine-model

Harvesting an Inguinal Draining Lymph Node from a Murine Model

Source: Rezende, R. M. et al., Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy.&#160;J. Vis. Exp.&#160; ...

a dissection method to harvest murine popliteal draining lymph nodes for ex vivo imaging

a-dissection-method-to-harvest-murine-popliteal-draining-lymph-nodes

A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging

Source: Rezende, R. M., et al. Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy. J. Vis. Exp. (2019)
This ...

in vitro culture and differentiation of primary monocytes into macrophages

in-vitro-culture-differentiation-primary-monocytes-into

In Vitro Culture and Differentiation of Primary Monocytes into Macrophages

Source: Plaisance-Bonstaff, K., et al. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (2019)
This video demonstrates the ...

differentiation of human-induced pluripotent stem cells into macrophages

differentiation-human-induced-pluripotent-stem-cells-into

Differentiation of Human-Induced Pluripotent Stem Cells into Macrophages

Source: Lopez-Yrigoyen, M., et al. Production and Characterization of Human Macrophages from Pluripotent Stem Cells. J. Vis. Exp. (2020).
This video ...

differentiation and polarization of monocyte-derived cells into macrophage-like cells

differentiation-polarization-monocyte-derived-cells-into-macrophage

Differentiation and Polarization of Monocyte-Derived Cells into Macrophage-Like Cells

in vitro generation of plasmacytoid dendritic cells from common lymphoid progenitors

in-vitro-generation-plasmacytoid-dendritic-cells-from-common-lymphoid

In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors

Source: Chang, S., et al. In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System. J. ...

generation of immature, mature, and tolerogenic dendritic cells from monocytes

generation-immature-mature-tolerogenic-dendritic-cells-from

Generation of Immature, Mature, and Tolerogenic Dendritic Cells from Monocytes

Source: Sim, W. J. et al., Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes.&#160;J. Vis. ...

in vitro mouse bone marrow stromal cell differentiation into adipocytes

in-vitro-mouse-bone-marrow-stromal-cell-differentiation-into

In Vitro Mouse Bone Marrow Stromal Cell Differentiation into Adipocytes

Source: Maridas, D. E. et al., Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice.&#160;J. Vis. ...

an in vitro method for the differentiation of megakaryocytes and platelet formation

an-vitro-method-for-differentiation-megakaryocytes-platelet

An In Vitro Method for the Differentiation of Megakaryocytes and Platelet Formation

Source: Perdomo, J., et al. Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells. J. Vis. Exp. (2017)
This video ...

three-dimensional thymic culture system to generate ipsc-derived thymic emigrants

three-dimensional-thymic-culture-system-to-generate-ipsc-derived

Three-Dimensional Thymic Culture System to Generate iPSC-Derived Thymic Emigrants

Source: Vizcardo, R., et al. A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific ...

purification and activation of platelets from murine whole blood

purification-and-activation-of-platelets-from-murine-whole-blood

Purification and Activation of Platelets from Murine Whole Blood

Source: Narciso, M. G., et al. Purification of Platelets from Mouse Blood. J. Vis. Exp. (2019).
In this video, we describe a protocol for purifying ...

an in vitro technique to generate human neutrophil extracellular traps

an-in-vitro-technique-to-generate-human-neutrophil-extracellular-traps

An In Vitro Technique to Generate Human Neutrophil Extracellular Traps

Source: Najmeh, S., et al. Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling. J. Vis. Exp. (2015)
This video demonstrates an ...

an in vitro technique to differentiate gm-csf producing t helper cells from na&iuml;ve t cells

an-vitro-technique-to-differentiate-gm-csf-producing-t-helper-cells

An In Vitro Technique to Differentiate GM-CSF Producing T Helper Cells from Na&iuml;ve T Cells

Source: Lu, Y., et al.&#160;In Vitro&#160;Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) ...

mast cell degranulation assay to study the effect of a target chemical

mast-cell-degranulation-assay-to-study-the-effect-of-a-target-chemical

Mast Cell Degranulation Assay to Study the Effect of a Target Chemical

flow cytometry-based cell sorting for different circulatory b cell populations

flow-cytometry-based-cell-sorting-for-different-circulatory-b-cell

Flow Cytometry-Based Cell Sorting for Different Circulatory B Cell Populations

functional assessment of antibody-secreting b cells using elispot assay

functional-assessment-antibody-secreting-b-cells-using-elispot

Functional Assessment of Antibody-Secreting B Cells Using ELISpot Assay

identifying regulatory t cell subsets from murine pancreatic draining lymph nodes

identifying-regulatory-t-cell-subsets-from-murine-pancreatic-draining

Identifying Regulatory T Cell Subsets from Murine Pancreatic Draining Lymph Nodes

Source: Luo, Z., et al. Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry. J. ...

immunological synapse formation between helper t cells and antigen-presenting cells

immunological-synapse-formation-between-helper-t-cells-antigen

Immunological Synapse Formation Between Helper T Cells and Antigen-Presenting Cells

Source: Bello-Gamboa, A. et al., Imaging the Human Immunological Synapse.&#160;J. Vis. Exp.&#160;(2019) This video demonstrates the in vitro study of ...

cd marker recognition of human cytokine-induced killer cells by flow cytometry

cd-marker-recognition-human-cytokine-induced-killer-cells-flow

CD Marker Recognition of Human Cytokine-Induced Killer Cells by Flow Cytometry

Source: Hsiao, C. H., et al. Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment. J. Vis. Exp. (2020)
In this video, ...

assay for the identification of interferon-gamma secretion in murine lymphoid organs

assay-for-identification-interferon-gamma-secretion-murine-lymphoid

Assay for the Identification of Interferon-Gamma Secretion in Murine Lymphoid Organs

Source: Mazet, J. M., et al. Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection. J. Vis. Exp. ...

characterization of macrophage extracellular traps in mouse lung tissue samples

characterization-macrophage-extracellular-traps-mouse-lung-tissue

Characterization of Macrophage Extracellular Traps in Mouse Lung Tissue Samples

Source: Sharma, R., et al. Visualizing Macrophage Extracellular Traps Using Confocal Microscopy. J. Vis. Exp. (2017)
In this video, we describe a protocol ...

preparing an antigen-adjuvant emulsion to induce autoimmune encephalomyelitis

preparing-an-antigen-adjuvant-emulsion-to-induce-autoimmune

Preparing an Antigen-Adjuvant Emulsion to Induce Autoimmune Encephalomyelitis

Source: B&#228;ckstr&#246;m, B. T. A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental ...

intravital visualization of gamma delta intraepithelial lymphocytes

intravital-visualization-of-gamma-delta-intraepithelial-lymphocytes

Intravital Visualization of Gamma Delta Intraepithelial Lymphocytes

Source: Jia, L., et al. Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine.&#160;J. Vis. Exp.&#160;(2019)
This video demonstrates ...

isolation of microparticles derived from apoptotic t lymphocytes in vitro

isolation-microparticles-derived-from-apoptotic-t-lymphocytes

Isolation of Microparticles Derived from Apoptotic T Lymphocytes In Vitro

Source: Yang, C. et al., Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells. J. Vis. Exp. ...

Encyclopedia Of Experiments - immunology