Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.

Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the sample surface area and provide a more efficient analysis. After grinding, the sample is sieved and mixed to ensure a uniform composition. The powdered sample is then dissolved in a suitable solvent to extract the analyte from the solid matrix. The solvent choice depends on the analyte's nature and the solubility characteristics of the sample components.

Liquid samples that contain suspended solid particles or precipitates are conventionally subjected to filtration or centrifugation to separate the solid impurities from the liquid. Filtration involves passing the liquid through a filter, while centrifugation uses centrifugal (spinning) force to separate the solids from the liquid phase. If the analyte is present in trace amounts, the liquid samples are subjected to pretreatment of concentration steps to increase the analyte concentration for better detection.

Sample preparation is usually unnecessary for analyzing gaseous analytes as the gas sample can be directly introduced into the analytical instrument.

Dal capitolo 9:

article

Now Playing

9.14 : Sample Preparation for Analysis: Overview

Method Development and Sampling Techniques

111 Visualizzazioni

article

9.1 : Sviluppo di metodi analitici

Method Development and Sampling Techniques

225 Visualizzazioni

article

9.2 : Controllo di qualità

Method Development and Sampling Techniques

92 Visualizzazioni

article

9.3 : Garanzia di qualità

Method Development and Sampling Techniques

70 Visualizzazioni

article

9.4 : Convalida dei dati

Method Development and Sampling Techniques

89 Visualizzazioni

article

9.5 : Analisi qualitativa

Method Development and Sampling Techniques

115 Visualizzazioni

article

9.6 : Analisi quantitativa

Method Development and Sampling Techniques

161 Visualizzazioni

article

9.7 : Calibrazione dello strumento

Method Development and Sampling Techniques

106 Visualizzazioni

article

9.8 : Calibrazione della vetreria

Method Development and Sampling Techniques

103 Visualizzazioni

article

9.9 : Soluzioni standard

Method Development and Sampling Techniques

117 Visualizzazioni

article

9.10 : Soluzioni in pezzi grezzi

Method Development and Sampling Techniques

123 Visualizzazioni

article

9.11 : Metodi di campionamento: Panoramica

Method Development and Sampling Techniques

157 Visualizzazioni

article

9.12 : Metodi di campionamento: Tipi di campione

Method Development and Sampling Techniques

129 Visualizzazioni

article

9.13 : Piani di campionamento

Method Development and Sampling Techniques

131 Visualizzazioni

article

9.15 : Preparazione dei campioni per l'analisi: tecniche avanzate

Method Development and Sampling Techniques

141 Visualizzazioni

See More

JoVE Logo

Riservatezza

Condizioni di utilizzo

Politiche

Ricerca

Didattica

CHI SIAMO

Copyright © 2025 MyJoVE Corporation. Tutti i diritti riservati