Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

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09:44 min

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July 10th, 2018

DOI :

10.3791/57270-v

July 10th, 2018

•

14,285 Views

Shih-Yun Chen¹, Hsiao-Ying Kuo¹, Fu-Chin Liu^1,2

¹Institute of Neuroscience, National Yang-Ming University, ²Brain Research Center, National Yang-Ming University

Capitoli

0:04

Title

0:40

Preparing the Microinjection Apparatus

4:09

Microinjecting Neonatal Mice

7:30

Results: Analysis of Injection of Cre-expressing Virus into P2 Striatum

9:03

Conclusion

Trascrizione

This method can help answer key questions in the genetic and neuro development such as during postnatal development. The main advantage of this technique is that it is a simple technique with home-made device. Visual demonstration of this method i

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Riepilogo

We describe a protocol of stereotaxic surgery with a homemade head-fixed device for microinjecting reagents into the striatum of neonatal mouse brains. This technique allows genetic manipulation in neuronal cells of specific regions of neonatal mouse brains.

Esplora Altri Video

Keywords Stereotaxic Surgery

Genetic Manipulation

Striatal Cells

Neonatal Mouse Brains

Postnatal Development

Tissue embedding Cassette

Stainless Steel Needles