Sensor Cartridge and Extracellular Flux Analyzer Calibrant, Concanavalin A (ConA)-Coated Microplate, and Sperm Buffer Preparation
1:52
Cartridge Calibration Preparation and Wave Template Generation
3:00
Mouse Sperm Isolation
3:42
Sensor Cartridge Loading
4:59
Sperm Plate Preparation
6:17
Results: Representative Changes in Glycolysis and Oxidative Phosphorylation During Mouse Sperm Capacitation
7:42
Conclusion
Trascrizione
This protocol can be used to apply an extracellular flux analyzer to monitor changes in energy metabolism during sperm capacitation. We developed this protocol to compare changes in glycolysis and oxidative phosphorylation in real time between non
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We describe the application of an extracellular flux analyzer to monitor real-time changes in glycolysis and oxidative phosphorylation during mouse sperm capacitation.