Sample Washes, Secondary Antibody Incubation, Ligation, Amplification, and Mounting
3:49
Digital Imaging with a Confocal Microscope
5:49
Image Analysis
7:41
Results: Increased Synapse Formation by Rapid Antidepressants in the Presence of GABAB Agonists
8:42
Conclusion
Trascrizione
DetectSyn takes advantage of the proximity ligation assay technology to quickly identify changes to synapses. We also provide a protocol to analyze the results. The main advantage of DetectSyn is how quickly the results can be obtained in the larg
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DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.