Preparation of Platelet-Rich Plasma from Human Peripheral Blood

Begin with human peripheral blood in a conical tube.

Centrifuge the tube at a low speed to separate the blood into three layers: red blood cells at the bottom, the buffy coat in the middle, and platelet-containing plasma at the top.

Transfer the plasma layer to a fresh tube and centrifuge it at a high speed to pellet the platelets. 

Transfer the plasma supernatant to a new tube.

Collect the platelet pellet and reconstitute it with an appropriate volume of the plasma supernatant to obtain platelet-rich plasma or PRP, which contains a high concentration of platelets.