Karen M. Polizzi

Simulation modeling of pooling for combinatorial protein engineering.

Pooling for improved screening of combinatorial libraries for directed evolution.

Structure-guided consensus approach to create a more thermostable penicillin G acylase.

High-throughput screening for enhanced protein stability.

Better library design: data-driven protein engineering.

Stability of biocatalysts.

Revealing biases inherent in recombination protocols.

Metabolic network reconstruction: advances in in silico interpretation of analytical information.

Genome-based kinetic modeling of cytosolic glucose metabolism in industrially relevant cell lines: Saccharomyces cerevisiae and Chinese hamster ovary cells.

The role of ER stress-induced apoptosis in neurodegeneration.

Protein construct optimization: data sharing strategy.

Binding of human BiP to the ER stress transducers IRE1 and PERK requires ATP.

In situ monitoring of intracellular glucose and glutamine in CHO cell culture.

What is synthetic biology?

An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells.

Comparative analysis of amino acid metabolism and transport in CHO variants with different levels of productivity.

Opportunities for bioprocess monitoring using FRET biosensors.

Can too many copies spoil the broth?

Towards controlling the glycoform: a model framework linking extracellular metabolites to antibody glycosylation.

Co-culture systems and technologies: taking synthetic biology to the next level.

Genetically-encoded biosensors for monitoring cellular stress in bioprocessing.

How does mild hypothermia affect monoclonal antibody glycosylation?

Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris.

A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation.

Model-based investigation of intracellular processes determining antibody Fc-glycosylation under mild hypothermia.

Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.

Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris.

Sense and sensitivity in bioprocessing-detecting cellular metabolites with biosensors.

Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation.

A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris.

A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization.

Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria.

Computational Re-design of Synthetic Genetic Oscillators for Independent Amplitude and Frequency Modulation.

Investigating the consequences of asymmetric endoplasmic reticulum inheritance in under stress using a combination of single cell measurements and mathematical modelling.

Tools for engineering coordinated system behaviour in synthetic microbial consortia.

Biosensor-assisted engineering of a high-yield Pichia pastoris cell-free protein synthesis platform.

Whole-Cell Biosensor with Tunable Limit of Detection Enables Low-Cost Agglutination Assays for Medical Diagnostic Applications.

Low-cost and user-friendly biosensor to test the integrity of mRNA molecules suitable for field applications.

BeQuIK (Biosensor Engineered Quorum Induced Killing): designer bacteria for destroying recalcitrant biofilms.

A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure.

Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Cell-Free System.

Improving the reaction mix of a cell-free system using a design of experiments approach to minimise experimental effort.

Microbial interventions are an easier alternative to engineer higher organisms.

Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System.

Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.

Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform.

Refactoring of a synthetic raspberry ketone pathway with EcoFlex.

Exploring conformational preferences of proteins: ionic liquid effects on the energy landscape of avidin.

An experimental approach probing the conformational transitions and energy landscape of antibodies: a glimmer of hope for reviving lost therapeutic candidates using ionic liquid.

Correction to: Refactoring of a synthetic raspberry ketone pathway with EcoFlex.

Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics.

Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics.

GenoChemetic Strategy for Derivatization of the Violacein Natural Product Scaffold.

High-yield 'one-pot' biosynthesis of raspberry ketone, a high-value fine chemical.

Expression of proteins in Pichia pastoris.

Cell-free protein synthesis using Chinese hamster ovary cells.

Model Identifies Genetic Predisposition of Alzheimer's Disease as Key Decider in Cell Susceptibility to Stress.

Production and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine.

Cell-Free Protein Synthesis Using Pichia pastoris.