in-situ-hybridization-for-sipunculus-nudus-coelomic-fluid

In situ Hybridization for <i>Sipunculus nudus</i> Coelomic Fluid

In situ hybridization (ISH) is a very informative technique to present cellular distribution patterns of specific genes (e.g., mRNA and ncRNA) in tissues. ...

Huaqiao University

huaqiao university

Deltamethrin (DM) is a widely used insecticide and reveals neural, cardiovascular and reproductive toxicity to various aquatic organisms. It has been known that DM negatively affects motion of zebrafish (Danio rerio). However, little is known in relation to the impacts of DM on development of swim bladder, which is a key organ for motion. In the present study, zebrafish embryos were exposed to 20 and 40 µg/L DM. The changes of swim bladder morphology were observed and transcription levels of key genes were compared between DM treatments and the control. The results showed that DM treatments significantly blocked the formation of progenitor and tissue layers in swim bladder of zebrafish embryos, leading to failed inflation of swim bladder. Compared with the control, the key genes (pbx1, foxA3, mnx1, has2, anxa5b, hprt1l and elovl1a) responsible for swim bladder development also showed decreased levels in response to DM treatments, suggesting that DM might specifically affect swim bladder development. Moreover, transcription levels of genes in the Wnt (wnt5b, tcf3a, wnt1, wnt9b, fzd1, fzd3 and fzd5) and Hedgehog (ihhb, ptc1 and ptc2) signaling pathways all decreased significantly in response to DM treatments, compared with the control. Considering the importance of Wnt and Hedgehog pathways in development of swim bladder, these results suggested that DM might affect swim bladder development through inhibiting the Wnt and Hedgehog pathways. Overall, the present study reported that swim bladder might be a potential target organ of DM toxicity in zebrafish, which contributed more information to the evaluation of DM's environmental risks.

Wenhua Li

Key Laboratory of Xiamen Marine and Gene Drugs,
School of Biomedical Sciences,
Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences

Huaqiao University

In situ Hybridization for Sipunculus nudus Coelomic Fluid

Wenhua Li

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Key Laboratory of Xiamen Marine and Gene Drugs,School of Biomedical Sciences,Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences

Huaqiao University

In situ Hybridization for Sipunculus nudus Coelomic Fluid

Key Laboratory of Xiamen Marine and Gene Drugs,
School of Biomedical Sciences,
Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences