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A*STAR 61 Biopolis Drive

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Assessing Antibody-dependent, Cell-mediated Cytotoxicity in Cancer Cells using Antibody-Dependent Cell-Mediated Cytotoxicity Reporter Bioassay

Kelvin Kam Yew Kuan^*1, Ke Zhang^*1,2, Koon Hwee Ang¹, Min Thura¹, Wei Hui Zheng^3,4

¹Institute of Molecular and Cell Biology (IMCB), A*STAR 61 Biopolis Drive, ²Department of Radiation Oncology, Affiliated Hangzhou Cancer Hospital, ³Key Laboratory of Head and Neck Cancer Translational Research of Zhejiang Province, Zhejiang Cancer Hospital, ⁴Department of Thyroid Surgery of Zhejiang Cancer Hospital

Here, we present a protocol for an antibody-dependent, cell-mediated cytotoxicity (ADCC) assay using an ADCC bioassay kit. This method offers a valuable tool for elucidating the ADCC mechanism and evaluating the therapeutic potential of antibodies in cancer immunotherapy.