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NYU Langone Medical Center

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy

Rachel Lubong Sabado¹, Elizabeth Miller², Meredith Spadaccia¹, Isabelita Vengco¹, Farah Hasan¹, Nina Bhardwaj¹

¹Cancer Institute, NYU Langone Medical Center, ²Infectious Diseases, NYU Langone Medical Center

The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.

Immunology and Infection

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Melissa V. Fernandez¹, Elizabeth A. Miller², Nina Bhardwaj³

¹Department of Pathology, New York University School of Medicine, ²Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, ³Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β expression.

Medicine

A Protocol for the Use of Remotely-Supervised Transcranial Direct Current Stimulation (tDCS) in Multiple Sclerosis (MS)

Margaret Kasschau^1,2, Kathleen Sherman^1,2, Lamia Haider², Ariana Frontario^1,2, Michael Shaw^1,2, Abhishek Datta³, Marom Bikson⁴, Leigh Charvet^1,2

¹Multiple Sclerosis Comprehensive Care Center, Department of Neurology, NYU Langone Medical Center, ²Department of Neurology, Stony Brook Medicine, ³Soterix Medical, Inc, ⁴Department of Biomedical Engineering, The City College of New York

The goal of this pilot study is to describe a protocol for the remotely-supervised delivery of transcranial direct current stimulation (tDCS) so that the procedure maintains standards of in-clinic practice, including safety, reproducibility, and tolerability. The feasibility of this protocol was tested in participants with multiple sclerosis (MS).

Neuroscience

Remotely Supervised Transcranial Direct Current Stimulation: An Update on Safety and Tolerability

Michael T. Shaw¹, Margaret Kasschau², Bryan Dobbs¹, Natalie Pawlak¹, William Pau¹, Kathleen Sherman¹, Marom Bikson³, Abhishek Datta⁴, Leigh E. Charvet¹

¹New York University, Langone Medical Center, ²Stony Brook Medicine, ³City College of New York, ⁴Soterix Medical

This manuscript provides an updated remote supervision protocol that enables participation in transcranial direct current stimulation (tDCS) clinical trials while receiving treatment sessions from home. The protocol has been successfully piloted in both patients with multiple sclerosis and Parkinson's disease.