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University at Buffalo

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Maxwell T. Koobatian¹, Carmon Koenigsknecht², Sindhu Row³, Stelios Andreadis³, Daniel Swartz²

¹Department of Physiology & Bio-Physics, State University of New York Buffalo School of Medicine, ²Department of Pediatrics, State University of New York Buffalo School of Medicine, ³Department of Chemical and Biological Engineering, State University of New York Buffalo School of Engineering

A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.

Behavior

Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats

Robert J. Wickham¹, Jinwoo Park², Eric J. Nunes³, Nii A. Addy^1,3,4

¹Interdepartmental Neuroscience Program, Yale University, ²Department of Biotechnical and Clinical Laboratory Sciences, School of Medicine and Biomedical Sciences, University at Buffalo, ³Department of Psychiatry, Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology, Yale School of Medicine

Rapid fluctuations in extracellular dopamine (DA) mediate both reward processing and motivated behavior in mammals. This manuscript describes the combined use of fast scan cyclic voltammetry (FSCV) and intra-oral tastant administration to determine how tastants alter rapid dopamine release in awake, freely moving rats.

Medicine

The Perinatal Asphyxiated Lamb Model: A Model for Newborn Resuscitation

Payam Vali^*1, Sylvia Gugino^*2, Carmon Koenigsknecht², Justin Helman², Praveen Chandrasekharan², Munmun Rawat², Satyan Lakshminrusimha¹, Jayasree Nair²

¹Department of Pediatrics, University of California Davis, ²Department of Pediatrics, University at Buffalo

Invasive instrumentation of the fetal lamb provides accurate physiologic measurements of the transitioning circulation in a model that closely mimics the newly born infant.

Immunology and Infection

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Andrew D. McCall¹, Mira Edgerton¹

¹Department of Oral Biology, University at Buffalo

We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images.

Behavior

Use of a Wireless Video-EEG System to Monitor Epileptiform Discharges Following Lateral Fluid-Percussion Induced Traumatic Brain Injury

Matthew J. McGuire¹, Steven M. Gertz¹, Jolie D. McCutcheon², Chelsea R. Richardson³, David J. Poulsen¹

¹Department of Neurosurgery, Jacobs School of Medicine and Biomedical Science, ²Comparative Medicine Laboratory Animal Facilities, University at Buffalo, ³emka Technologies

Here we present a protocol to induce severe TBI with the lateral fluid percussion injury (FPI) model in adult, male Wistar rats. We also demonstrate the use of a wireless telemetry system to collect continuous video-EEG recordings and monitor for epileptiform discharges consistent with post-traumatic epileptogenesis.