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Temasek Polytechnic

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Yanfen Peng¹, Victor Van Gelder², Anburaj Amaladoss¹, Kadamb Haribhai Patel¹

¹School of Applied Science, Temasek Polytechnic, ²University of Applied Sciences Utrecht

In this manuscript, periodate oxidation and glutaraldehyde cross-linking methods for covalent immobilization of antibodies on paper discs are presented. The binding activity of immobilized antibodies was evaluated. Based on these results, a glutaraldehyde cross-linking method was used to develop a novel paper-based immunoassay for immunoglobulin G (IgG) detection.

Medicine

The Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat

Kum Fai Chooi¹, Dinesh Babu Kuppan Rajendran¹, Siew Siang Gary Phang¹, Han Hui Alden Toh¹

¹Technology Development, School of Applied Science, Temasek Polytechnic

We describe a method to produce an animal model of liver fibrosis in the rat, and assess the degree of fibrosis by histological examination of the liver. The model can be used to study the development of liver disease as well as to test the efficacy of potential anti-fibrotic agents.