Temasek Polytechnic
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
Yanfen Peng 1, Victor Van Gelder 2, Anburaj Amaladoss 1, Kadamb Haribhai Patel 1
1School of Applied Science, Temasek Polytechnic, 2University of Applied Sciences Utrecht
In this manuscript, periodate oxidation and glutaraldehyde cross-linking methods for covalent immobilization of antibodies on paper discs are presented. The binding activity of immobilized antibodies was evaluated. Based on these results, a glutaraldehyde cross-linking method was used to develop a novel paper-based immunoassay for immunoglobulin G (IgG) detection.
Medicine
The Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat
Kum Fai Chooi 1, Dinesh Babu Kuppan Rajendran 1, Siew Siang Gary Phang 1, Han Hui Alden Toh 1
1Technology Development, School of Applied Science, Temasek Polytechnic
We describe a method to produce an animal model of liver fibrosis in the rat, and assess the degree of fibrosis by histological examination of the liver. The model can be used to study the development of liver disease as well as to test the efficacy of potential anti-fibrotic agents.
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