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Fujita Health University

8 ARTICLES PUBLISHED IN JoVE

Biology

¹Genetic Engineering and Functional Genomics Unit, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University

The light/dark transition test is one of the most widely used tests to measure anxiety-like behavior in mice. Here, we present a movie that shows detailed procedures on how we conduct the test.

Biology

Elevated Plus Maze for Mice

Munekazu Komada¹, Keizo Takao^1,2, Tsuyoshi Miyakawa²

¹Genetic Engineering and Functional Genomics Unit, Frontier Technology Center, Graduate School of Medicine, Kyoto University, ²Institute for Comprehensive Medical Science Division of Systems Medicine, Fujita Health University

The elevated plus maze test is one of the most widely used tests for measuring anxiety-like behavior in mice. Here, we present a movie showing the detailed procedures for conducting the test.

Biology

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Hideo Hagihara^1,2, Keiko Toyama^1,2, Nobuyuki Yamasaki^1,3, Tsuyoshi Miyakawa^1,2,4,5

¹Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), ²Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, ³Department of Psychiatry, Graduate School of Medicine, Kyoto University, ⁴Genetic Engineering and Functional Genomics Group, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, ⁵Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

A dissection technique for removal of the dentate gyrus from adult mouse under a stereomicroscope was demonstrated in this video-recorded protocol.

Neuroscience

T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice

Hirotaka Shoji^1,2, Hideo Hagihara¹, Keizo Takao³, Satoko Hattori^1,2,3, Tsuyoshi Miyakawa^1,2,3

¹Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, ²Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), ³Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

This article presents the protocol of T-maze tests using a modified automated apparatus for assessing the learning and memory functions in mice.

Behavior

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

Hirotaka Shoji^1,2, Keizo Takao^2,3, Satoko Hattori^1,2, Tsuyoshi Miyakawa^1,2,3

This article presents a protocol for a contextual and cued fear conditioning test using a video analyzing system to assess fear learning and memory in mice.

Behavior

Clinical-oriented Three-dimensional Gait Analysis Method for Evaluating Gait Disorder

Masahiko Mukaino¹, Kei Ohtsuka², Hiroki Tanikawa², Fumihiro Matsuda², Junya Yamada³, Norihide Itoh⁴, Eiichi Saitoh¹

¹Department of Rehabilitation Medicine, School of Medicine, Fujita Health University, ²Faculty of Rehabilitation, School of Health Science, Fujita Health University, ³Department of Rehabilitation, Fujita Health University Hospital, ⁴Department of Advanced Rehabilitation Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

In this study, a clinician-friendly three-dimensional gait analysis method, which was designed to be performed in the rehabilitation clinic, is presented. The method consists of a simplified measurement method and intuitive figures to facilitate clinicians' understanding of the results.

Biology

Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Yuka Mizutani¹, Daisuke Kuga², Machiko Iida¹, Kaori Ushida³, Tsuyoshi Takagi¹, Yoshihito Tokita¹, Masahide Takahashi³, Masato Asai^1,3

¹Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, ²Surgery Department, Anjo Kosei Hospital, ³Department of Pathology, Nagoya University Graduate School of Medicine

Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies.

Cancer Research

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Hiroki Kobayashi^1,2,3,4, Krystyna A. Gieniec^1,2, Jia Q. Ng^1,2, Jarrad Goyne^1,2, Tamsin R. M. Lannagan^1,2, Elaine M. Thomas^1,2, Georgette Radford^1,2, Tongtong Wang^1,2, Nobumi Suzuki^1,2,5, Mari Ichinose^1,2, Josephine A. Wright², Laura Vrbanac^1,2, Alastair D. Burt⁶, Masahide Takahashi^3,4,7, Atsushi Enomoto³, Daniel L. Worthley², Susan L. Woods^1,2

¹Adelaide Medical School, University of Adelaide, ²South Australian Health and Medical Research Institute (SAHMRI), ³Department of Pathology, Nagoya University Graduate School of Medicine, ⁴Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, ⁵Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, ⁶Translational and Clinical Research Institute, Newcastle University, ⁷International Center for Cell and Gene Therapy, Fujita Health University

Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.