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Boston University Photonics Center

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

In vivo Neuronal Calcium Imaging in C. elegans

Samuel H. Chung^*1,2, Lin Sun^*1,2, Christopher V. Gabel^1,2

¹Department of Physiology and Biophysics, Boston University School of Medicine, ²Boston University Photonics Center

With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.

Immunology and Infection

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Lin Sun¹, Naoko Kono², Hiroyuki Toh³, Hanbing Xue¹, Kaori Sano^4,5, Tadaki Suzuki⁴, Akira Ainai⁴, Yasuko Orba⁶, Junya Yamagishi^7,8, Hideki Hasegawa^4,5, Yoshimasa Takahashi⁹, Shigeyuki Itamura², Kazuo Ohnishi^9,10

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Center for Influenza Virus Research, National Institute of Infectious Diseases, ³School of Science and Technology, Kwansei Gakuin University, ⁴Department of Pathology, National Institute of Infectious Diseases, ⁵Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, ⁶Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, ⁷Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, ⁸Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, ⁹Department of Immunology, National Institute of Infectious Diseases, ¹⁰Faculty of Life and Environmental Sciences, University of Tsukuba

Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.

Medicine

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration

Jin Wang^*1, Wenjia Li^*2, Yao He¹, Yuanbin Xia¹, Lin Sun¹, Youpeng Zhang¹, Zhaohui Zhu¹

¹Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ²Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

This protocol presents the clinical application of a 24 G cannula and 3-0 polypropylene suture as a simple and effective method for the exploration of the vas deferens.