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Helmholtz Centre for Environmental Research - UFZ

2 ARTICLES PUBLISHED IN JoVE

Environment

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Susan Schamfuß¹, Thomas R. Neu², Hauke Harms¹, Lukas Y. Wick¹

¹Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, ²Department of River Ecology, Helmholtz Centre for Environmental Research - UFZ

A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.

Environment

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Johannes Lambrecht¹, Florian Schattenberg¹, Hauke Harms¹, Susann Mueller¹

¹Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ

Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.