Helmholtz Centre for Environmental Research - UFZ
2 ARTICLES PUBLISHED IN JoVE
Environment
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
Susan Schamfuß 1, Thomas R. Neu 2, Hauke Harms 1, Lukas Y. Wick 1
1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, 2Department of River Ecology, Helmholtz Centre for Environmental Research - UFZ
A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.
Environment
Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
Johannes Lambrecht 1, Florian Schattenberg 1, Hauke Harms 1, Susann Mueller 1
1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ
Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.
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